世界*實驗材料供應商 Genetex 正式授權上海起發為其中國代理， Genetex 在一直是行業的*,一直為廣大科研客戶提供zui為的產品和服務,上海起發一直秉承為中國科研客戶帶來的產品，的服務，簽約 Genetex 就是為了給廣大科研客戶帶來更加完善的產品和服務,您的滿意將是我們zui大的收獲
Genetex 中國代理, Genetex 上海代理， Genetex 北京代理，Genetex 廣東代理， Genetex 江蘇代理Genetex 湖北代理,Genetex 天津,Genetex 黑龍江代理,Genetex 內蒙古代理,Genetex 吉林代理,Genetex 福建代理, Genetex 江蘇代理, Genetex 浙江代理, Genetex 四川代理,
GeneTex 公司于1997年成立于美國得克薩斯州的圣安東尼奧（現已遷至南加州），是由三位的科學家創立，公司致力于研究和開發各種基礎醫學相關的新型研究用抗體。公司起初的核心目標是幫助科學家尋找引發人類乳腺癌的分子新靶標，現在已經逐步擴展到其他研究領域新抗體和配套診斷試劑的開發。至今，癌癥研究相關抗體已經成為了該公司的特色產品。
| 貨號： | gtx127375 |
| 應用范圍： | Flow cytometry/FACS, ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| 適應物種： | Human, Mouse, Pig, Rat |
| 宿主： | Rabbit |
| 克隆性： | 否 |
GeneTex為您尋找*替代抗體:
因近期越來越多客戶詢問其他廠家的替代產品, GeneTex本著服務客戶的精神, 特別推出以貨號查詢替代產品的功能, 詳情如下, 歡迎大家使用。
Santa Cruz 抗體替代產品：http://www.genetex。。ｃｏｍ/Web/LandingPage/LandingPageView.aspx?n=197
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:100.
Blue: Hoechst 33342 staining.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
HepG2 cells were untreated or treated with 3 μM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000.
LC3B antibody detects LC3B protein in HCV-infected samples by Western blot analysis.
A. 20 µg Huh7 whole cell lysate/extract (un-infected)
B. 20 µg Huh7 whole cell lysate/extract (HCV-infected)
LC3B antibody (GTX127375) dilution: 1:1500
LC3B antibody detects MAP1LC3B protein by Western blot analysis.
A. 20 µg Huh7 whole cell lysate/extract (untreated)
B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr)
LC3B antibody (GTX127375) dilution: 1:1500
LC3B antibody detects LC3B protein by western blot analysis. Un-treated (-) and treated (+, 50μM Chloroquine treatment for 48 hr) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) at a dilution of 1:500.The beta Actin was used as internal control (GTX10564, 1:50000) shown at the bottom panel.
LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000.
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 μg of LC3B antibody (GTX127375).
Western blot analysis was performed using LC3B antibody (GTX127375).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
LC3B antibody detects LC3B protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
LC3B antibody (GTX127375) diluted at 1:500.
LC3B antibody (GTX127375) detects LC3B protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: LC3B antibody (GTX127375) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
LC3B antibody detects MAP1LC3B protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000
LC3B antibody detects MAP1LC3B protein by Western blot analysis.
A. 50 µg Rat brain lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000
LC3B antibody detects LC3B protein in Thapsigargin-treated samples by Western blot analysis.
Upper panel: LC3B antibody (GTX127375)
Lower panel: Beta-actin antibody (GTX110564)A. 30 µg HepG2 whole cell lysate/extract (untreated)
B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr)
15 % SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000
Beta-actin antibody (GTX110564) dilution: 1:20000
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6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
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